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Western blots are frequently included in pharmaceutical research papers to report results when analysing proteins in a sample. However, research shows that Western blot images are a common source of integrity issues, the publication of which can be harmful to the advancement of new treatments. Here, Dr Dror Kolodkin Gal, founder of image checking tool, Proofig AI, explores the challenges faced when reviewing Western blot images in pharmaceutical research. Then, he advises on how to more effectively identify issues before publication to maintain the integrity of research that can improve health outcomes.